Bahman Mirzaei
Department of Microbiology, Microbial research center, Pasteur Institute of Iran, Tehran. Microbiology Research Center (MRC) Pasteur Institute of Iran
Title: Preparation and immunological characterization of conjugated PIA- SesC as candidate vaccine against biofilm forming Staphylococcus epidermidis
Biography
Biography: Bahman Mirzaei
Abstract
Staphylococcus epidermidis as an opportunistic pathogen and the leading cause of morbidity and premature mortality in patients with medical devices related infections, has been described as a concerning subject for hospitalized health. Developing a strategy to raise opsonic antibodies against polysaccharide intracellular adhesion (PIA) could be promising for elimination of colonization and biofilm forming Staphylococcus epidermidis. Following the purification of truncated SesC protein and mentioned polysaccharide, for the first time polysaccharide intracellular adhesion (PIA) was conjugated by using rSesC protein as a safe carrier for increasing the immunogenicity of polysaccharide and evaluated its efficacy in mice. Construction of conjugate was analyzed by using Fourier Transform Infrared spectroscopy (FTIR) and Proton Nuclear Magnetic Resonance spectroscopy (H1- NMR) methods. Subsequently, the immune response of total IgG, IgG2a, and IgG2b titers were assessed. Immunization of mice with the PIA-SesC conjugate raised the levels of opsonic antibodies, and the vaccinated mice were protected when challenged intravenously by wild type strain 1457. Further studies showed that the conjugated vaccine could eliminate S. epidermidis biofilm formation at the in vitro and in vivo assays. This study supports the proposal that immunization of mice with PIA-SesC conjugate vaccine could be safe and protective against Staphylococcus epidermidis infection.