Sanjiva Bimal
Division of Immunology, Rajendra Memorial Research Institute of Medical Sciences, Patna, 800007, India.
Title: Immunization with Leishmania donovani Protein disulfide isomerase DNA construct induces Th1 and Th17 dependent immune response and protection against experimental visceral leishmaniasis in Balb/c mice
Biography
Biography: Sanjiva Bimal
Abstract
In the present study the efficacy of L.donovani protein disulfide isomerase (LdPDI) as a DNA vaccine was evaluated in BALB/C mice. Mice immunized with LdPDI-DNA construct was found to be most immuno-reactive inducing higher T-cell proliferation which was associated with substantial rise in Th1 and Th17+ CD4 cell response as well as it triggered a higher proportion of CD8+ T cells for release of Interferon-gamma along with reduced splenic parasite load on day 20 and 60 post challenge (p.c). Further, the same vaccine construct triggered an increased IFN-γ, IL-17A and IL-22 release accompanied with decreased ERK1/2 signalling and increased MAPK signalling coincided with increase in amount of nitrite and ROS in vaccinated the splenocyts. We summarise from our data that DNA-PDI construct of Leishmania donovani has potential for eliciting protective immunity through pro-inflammatory cytokines of CD8+ and CD4+(Th1 and Th17) following an intervention in downstream signalling event of ERK1/2 probably through p38MAPK signalling . Therefore, the study suggests a new control against VL in future