8^th European Immunology Conference

Biography

Biography: Ali Ganji

Abstract

Background: Multiple sclerosis (MS) is an autoimmune, chronic, and inflammatory disease of the CNS, which threatens many people, annually. There has not been introduced any cure for the MS, yet. Due to the anti-inflammatory and antioxidant effects of walnut, determining the effects of the walnut oil on the MS may lead to understanding new supplements for the treatment of MS. In this study, we have investigated the therapeutic effects of walnut oil on EAE mouse model of the MS disease. Materials and Methods: EAE was induced in the inbred C57BL6 mice. The control and test groups were gavaged 100μL of walnut oil daily. The weight and clinical symptoms were monitored, daily, until the day 21st. The spleen and the brain of the mice were removed and used for ELISA and histological studies. Results: The average weight changes between all of the groups was not significant (p value > 0.05). The average maximum severity of the disease and plaque formation in the brain of walnut oil-treated group was significantly lower (p value < 0.05) than the control group. However, leukocyte infiltration and MTT assay result differences were not significant between the two groups. Cytokine measurements in spleen cell soup, stimulated with MOG protein, showed a significant decrease in INF-γ and IL-17 in the treatment group, but there were no significant differences in the levels of IL-10 and IL-5 between the two groups. Serum evaluations of these cytokines revealed a significant (p value <0.05) decrease and increase in IL-17 and IL-10 values, respectively. Conclusion: Walnut oil, significantly, has inhibited the plaque formation and has inactivated the infiltrated cells in the brain. Thus, this food can reduce the MS disease severity. More studies are required to introduce the walnut oil as a valuable supplement in the treatment of MS.