5^th European Immunology Conference

Biography

Biography: Mikhailova Valentina Anatolevna

Abstract

Preeclampsia is a complication of pregnancy that affects health of woman and fetus. Pathogenesis of preeclampsia is closely connected with inflammation in fetal-maternal interface. The aim of the study was to assess leucocyte phenotype and functions in case of healthy pregnancy and preeclampsia. Ethical Committee of FSBSI (The Research Institute of Obstetrics, Gynecology and Reproductology named after D.O.Ott), Saint-Petersburg, Russia, approved the study. Peripheral blood was obtained from 422 women (healthy non pregnant women, healthy pregnant women (38-39 w.g.) and pregnant women with preeclampsia (38-39 w.g.). Such methods as flow cytometry (BD FacsCanto II, USA), atomic force microscopy (NT-MTD, Russia) and cell cultures (THP-1, Ea.Hy926) were used. We showed that expression of CD11c byNK-cells, CD18 by CD8+T-cells and CD119 by monocytes increased in case of preeclampsia comparing with healthy pregnancy. So does in vitro adhesion of lymphocytes and monocytes to intact and TNFα activated endothelium. CD107a expression by NK cells was lower and TRAIL expression was higher in case of preeclampsia comparing with healthy pregnancy. Plasma of peripheral blood of all studied patients contained cellular microparticles (average size: 330 nm). In case of preeclampsia the amount of microparticles with receptors CD45 and CD16 increased comparing with healthy pregnancy. Microparticles from plasma of women with preeclampsia in vitro influenced expression of CD181, CD18 and CD54 by monocytic cells (THP-1). Observed changes in adhesion receptors expression, adhesion function of T-cells, NK cells and monocytes, TRAIL expression of NK cells are a result of inflammation in decidua, which can be triggered by cellular microparticles present in plasma.