Manal Mohamed Saber
Minia University, Egypt
Title: Therapeutic impact of EMAP II and CD13 expression in colorectal cancer
Biography
Biography: Manal Mohamed Saber
Abstract
Statement of the Problem: Colorectal cancer (CRC) is the third most commonly diagnosed cancer and a major cause of cancer mortality in the world. Despite advances in CRC treatment, a greater proportion of patients, highlighting the need for new immunotherapy. Endothelial monocyte-activating polypeptide-II (EMAP-II) is a multifunctional cytokine with pro-inflammatory properties. Aminopeptidase N (CD13) is a zinc-binding protease that has a role in cancerogenesis. It is proposed that both EMAP II and CD13 are involved in tumor progression and metastasis. The purpose of this study is to develop a novel immunotherapy expressing anti-EMAP and anti-CD13 antibodies in CRC.
Methodology & Theoretical Orientation: Expression of both EMAP II and CD13 was studied in CRC patients by immunohistochemistry, flow cytometry and ELISA. The antitumour effect of the anti-EMAP II and anti-CD13 antibodies was verified by therapeutic animal experiments in vivo.
Findings: There was a positive correlation between CD13 and EMAP II expression in CRC with accordingly decline in CD4 and CD8 cells. Coculture of peripheral T cells with recombinant EMAP II caused a significant increase in CD13+ T cells in comparison to control (P<0.001). In an animal model, an administration of anti-EMAP II and anti-CD13 antibodies resulted in extension of the survival of the mice compared to the untreated group. Furthermore, novel immunotherapy decreased CD13+T cells and increased CD4-positive T cells, CD8-positive T cells, NK cells. Furthermore, novel immunotherapy decreased the tumour-induced apoptosis of T cells.
Conclusion & Significance: This study has demonstrated a novel promising tumour immunotherapy for CRC.