Bakaeva Zanda Valerievna
People’s Friendship University of Russia, Russia
Title: Comparison of systemic and local cytokine profile on the background of development and healing of experimental ulcer
Biography
Biography: Bakaeva Zanda Valerievna
Abstract
Cytokines plays an important role in the development and course of diseases of different systems, including the digestive system. Lymphocytes of regional lymph nodes are the main sources of cytokines which are involved in inflammation. We compared systemic and local cytokine profile of rats with acetate ulcer (AU). Blood was collected from the jugular vein of the rats on the day 4 and 7 after acetate-induced ulceration. Max. AU – after 3 days, 10 days formed scar. ln. gastricus caudalis of rats were used as the source of T-lymphocytes. Cytokine’s levels were determined in the serum and in the supernatant of mitogen-activated mononuclear cells by flow cytometry. The max. concentration of IL-1α and MCP-1 in the serum of rats without ulcers (IR) was 61.9±15.1 pg/ml and 537.2±82.2 pg/ml resp. Other cytokines were <10 pg. The AU formation induced a significant increase of IL-1α and MCP-1 in the serum in 2.2 and 13.5 times. The level of the other cytokines did not change. The max. of IL-1α, MCP-1, IL-17α, IFNg, IL-4, TNFα in the supernatant of lymph node cells were equal to 273.0±24.6, 62.7±8.7, 2852.8±146.5, 1115.9±168.8, 598.9±33.8, 87.5±18.1 pg/m, resp. AU formation was accompanied by an acute shortage of all local cytokines. This continued on the day 7, expressly were low IL-1α and MCP-1, alone IL-17α reverted to normal. So, despite that the development and healing of AU were accompanied by high content in serum of basic proinflammatory cytokines, contrary local immunity is suffer from their deficiency. At help comes IL-17α.