Kim Do Won
Hallym University, Korea
Title: Immunological method for measuring LDL-cholesterol using the streptolysin O and apolipoprotein B-100 antibody
Biography
Biography: Kim Do Won
Abstract
Cholesterol is one of the important components for necessary to maintain the body. It is related to function as a component of cell membranes and it is also an important raw material of sex hormone and cortical hormone. For function of cholesterol, it needs movement through blood. Because cholesterol does not dissolve in water, it moves through lipoprotein in blood. The lipoproteins can be divided into LDL (Low Density Lipoprotein) and HDL (High Density Lipoprotein) depending on the density. When LDL-cholesterol is present in high amount in blood, wall of blood vessels will be covered with lipid component. Therefore, it is important to measure value of LDL-cholesterol and diagnose diseases such as Cardiovascular Disease (CVD), obesity, diabetes, atherosclerosis, hypertension and stroke. In this paper, it shows that measuring the LDL-cholesterol in blood through an immunological method. The paper suggests double bio marker, using two types of immunological measuring methods. One is streptolysin O (SLO) recombinant protein that it can specifically combine with cholesterol because carboxyl-terminal of SLO has tryptophan-rich. The other is a monoclonal antibody about Apolipoproetin B-100 existing in surface of LDL particle. The experimental results show that measuring LDL-cholesterol is dependent concentration and LDL- cholesterol of 2ãŽ/ml recognize the value of O.D. 630nm <0.77. Also, the method recognized concentration of cholesterol until 125 to 250ng/ml. For those reasons, this method can consider measuring LDL- cholesterol in serum.