Igor Malyshev
Moscow State University of Medicine and Dentistry, Russia
Title: Genetic features of NO generating systems and resistant to Ehrlich carcinoma
Biography
Biography: Igor Malyshev
Abstract
Introduction: An important factor of antitumor immune defence is macrophage NO. We hypothesized that the tumor vulnerability can be predetermined by genetic features of NO generating systems. Methods: The content of NO in tumor was changed by the iNOS inhibitor ITU, NO traps c-PTIO and NO donor. NO production was evaluated by nitrites. Macrophage phenotype was assessed by iNOS and CD markers. Results: The lifespan of mice C57BL/6N with carcinoma was 25% more than C57BL/6J. NO content reduction decreased lifespan of high-resistant to tumor subline C57BL/6N by 23%. NO content rise increased lifespan of low-resistant subline C57BL/6J by 26%. C57BL/6N M1 macrophages had a higher NO production, than C57BL/6J M2 macrophages. Discussion: Thus, the carcinoma vulnerability is determined by genetic features of macrophage NO generating systems. C57BL/6J and C57BL/6N have differences in SNP and NNT (nicotinamide nucleotide transhydrogenase) gene. NO, NNT and SNP deserve attention in developing methods for anticancer therapy.
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