Ahmed M Elbana
King Fahd Military Medical Complex, KSA
Title: Endogenous bone marrow stem cells mobilization in rats: Its potential role for homing and repair of the damaged inner ear
Biography
Biography: Ahmed M Elbana
Abstract
Objective:
This study assessed the possibility of mobilizing endogenous bone marrow derived stem cells (SCs) in rats using Granulocyte colony stimulating factor (G-CSF) to induce regeneration and repair to experimentally damaged inner ear hair cells induced in rats by intratympanic Amikacin injection.
Material & Methods:
The study included thirty adult Sprague Dawley male rats. Experimental induction of inner ear damage was done by repeated intratympanic injection of amikacin sulfate. Mobilization of bone marrow SCs was provoked by subcutaneous injection of GCSF. Cochlear integrity, induction of hearing loss and functional recovery of sensory hearing loss were assessed using Distortion Product Otoacoustic Emission (DPOAEs). The morphological alteration and recovery of the organ of Corti was assessed histologically using the light and scanning electron microscopes.
Results:
After six month duration, there was improvement in 50% of the sensorineural DPOAE results. Functional recovery coincided with the histological repair of structural components of organ of Corti.
Conclusion:
SCs mobilization by G-CSF is a promising alternative method for replacement therapy in sensorineural hearing loss.