Sthiti Porna Dutta
North Eastern Hill University, India
Title: Effects Of N-Nitrosodibutylamine on The Expression Of The Liver Mitochondrial Membrane Surface Proteins In Mice
Biography
Biography: Sthiti Porna Dutta
Abstract
Effects of N-Nitrosodibutylamine on the expression of the liver mitochondrial membrane surface proteins in mice: N-Nitrosodibutylamine (DBN) is an established hepatocarcinogen in rodents and its effects on liver mitochondria in Swiss albino mice have been examined. In the present investigation, DBN at a dosage of 10 mg kg-1 body weight was used to induce carcinogenesis in male mice by intravenous administration of the carcinogen at weekly intervals for a period of 16 weeks. This protocol triggered the initiation of carcinogenesis. Cancer induction was followed by monitoring the activities of marker enzymes such as acetylcholine esterase (AChE), glutathione-S-transferase (GST) and gamma-glutamyl transpeptidase(GGT), which was further supported by the histological examination of liver tissue of DBN exposed mice. The observed alteration in marker enzymes activities indicate hepatic dysfunction and damage to liver caused by DBN-treatment in mice. Whether, the DBN exposure also affect on mitochondrial membrane surface protein was of great concern to us. Hence liver mitochondria are chosen as our target of interest. Our preliminary observations indicate a drastic distortion in shape and size of liver mitochondria in mice upon DBN exposure. The membrane was highly disrupted and mitochondria contained large vacuoles or enlarged cristae compartments in comparison to the normal control mice. The proteomic analysis of the liver mitochondrial membrane surface proteins showed differential expression in DEN-treated mouse compared to that of the normal control. These results suggest that specific mitochondrial proteins are uniquely susceptible to alterations in expression and carry implications for the further investigation of their potential as therapeutic and prognostic markers.