Abdolkarim Sheikhi
Dezful University of Medical Sciences, Dezful, Iran
Title: Yogurt culture Lactobacilus delbrueckii subsp. Bulgaricus modulates the secretion of Th1/Th2 and Treg cell-related cytokines by PBMCs from patients with atopic dermatitis
Biography
Biography: Abdolkarim Sheikhi
Abstract
BACKGROUND: Atopic dermatitis (AD) is an inflammatory skin disease which may be due to the imbalance between Th1-, Th2 and Treg cell-related immune responses. Evidences suggest that appropriate stimulation with probiotics may correct the skewed immune response in children with AD. The aim was to determine the effects of the yogurt culture lactobacillus delbrueckii subsp. Bulgaricus on the secretion of Th1/Th2/Treg type cytokines by peripheral blood mononuclear cells (PBMCs) from children with AD.
METHODS: L. Bulgaricus was cultivated on MRS broth. The PBMCs from 20 children with AD were separated by Ficoll-Hypaque centrifugation and co-cultured with different concentrations of UV killed bacteria in RPMI-1640 plus 10% FCS for 48/72h. The levels of IL-10, IL-4, IL-12 and IFN-γ were measured in supernatant of PBMCs by ELISA. RNA extraction and real time RT-PCR were used to measure Foxp3 mRNA expression. RESULTS: L. Bulgaricus significantly up-regulated the secretion of IL-10, IL-12 and IFN-γ, whereas decreased the secretion of IL-4 by PBMCs compared to control (p<0.05). The secretion level of IL-10 by L. Bulgaricus-stimulated PBMCs at incubation time 72h was significantly higher than 48h. The expression of Foxp3 mRNA was changed accordingly (P<0.001 and P<0.01, respectively). CONCLUSION: These data show that L. Bulgaricus may modulate the secretion of Th1-, Th2- and Treg-related cytokines in AD patients. Therefore, the possible potential therapeutic of L. Bulgaricus for treatment of AD should be consider in further investigation.