Haiyan Xu
Third Affi liated Hospital of Soochow University, China
Title: Effects of B cells activating factor on growth characteristics of mouse renal tubular epithelial cells
Biography
Biography: Haiyan Xu
Abstract
Backgrounds: We previously found that BAFF and BAFF-R highly expressed on renal tubular epithelial cells (RTECs) in kidney allograft rejection tissues, but the significance of abnormal expression is still to be further investigated.
Methodology: Primary mouse RTECs (from 4-6 w C57BL/6) were isolated by enzyme digestion method, and cultured in the conditioned medium. After stimulated by 500U/mL IFN-γ for 48h, the expression of BAFF and BAFF-R on RTECs were detected by FCM. After treated with recombinant mouse BAFF and/or blockade BAFF-R-Fc fusion protein for 48h, the transport ability of fluorescein sodium and CK-18 expression of RTECs were detected, respectively; Proliferation ability and apoptosis rates of RTECs were tested by MTS and FCM method, respectively; Data were analyzed by SPSS17.0, and P < 0.05 was considered to be statistically significant. Results BAFF-R expression on RTECs in the IFN-γ treated group significantly up-regulated, compared with control group. Fluorescein sodium transport ability and CK-18 expression of BAFF stimulation group was significantly lower than that of BAFF-R-Fc+BAFF group and control group, respectively; Cell proliferation ability of 5ng/mL and 20ng/mL BAFF group were significantly higher than that of control group and BAFF-R-Fc+BAFF group; While apoptosis rate of BAFF-treated group was significantly lower than that of BAFF-R-Fc+BAFF group and control group.
Conclusion & Significance: BAFF/BAFF-R signaling could promote the proliferation ability of RTECs, but down-regulate the epithelial cell characteristics and Ionic transport ability of RTECs. And it is worth exploring whether enhancement of BAFF/BAFF-R signaling promotes the interstitial transformation of RTECs.